Dendritic spatial flicker of local membrane potential due to channel noiseand probabilistic firing of hippocampal neurons in culture

Whole-cell recordings and imaging of dissociated hippocampal neurons staine d with voltage sensitive dye provide a new microscopic picture or neuronal excitation. This is the first attempt to combine imaging of active channel clusters on the geometry of live neurons and a theoretical approach. During single somatic action potentials and the back-invasion into the neurites, local mean potentials are generated at sites of active channel clusters whi ch are unevenly distributed in the neuronal membrane. Similar mean membrane potentials are observed in the neurites and at the soma. Identical action potentials produce different spatial patterns of mean membrane potentials f rom trial to trial. This spatial variability is explained by the stochastic behavior of the channels in the clusters. When hippocampal neurons are exc ited by synaptic inputs, their evoked responses are probabilistic and gener ate variable spatial patterns of mean membrane potential trial after trial. Our stochastic model reproduces this random behavior by assuming that the voltage fluctuations generated by channel noise are added to the synaptic p otentials reaching the soma. We demonstrate that the probability of action potential initiation depends on the strength of the synaptic input. the dia meter of the dendrites and the relative positions of the channel clusters, of the synapse and of the soma. (C) 2001 Elsevier Science Ireland Ltd and t he Japan Neuroscience Society. All rights reserved.