Bisulfite genomic sequencing of microdissected cells

Mapping of methylation patterns in CpG islands has become an important tool for understanding tissue-specific gene expression in both normal and patho logical situations. However, the inherent cellular heterogeneity of any giv en tissues can affect the outcome and interpretation of molecular studies. In order to analyse genomic DNA methylation on a pure cell population from tissue sample, we have developed a simple technique of single-cell microdis section from cryostat sections which can be combined with bisulfite-mediate d sequencing of 5-methylcytosine. We report here our results on the methyla tion status of the androgen receptor gene studied by bisulfite genomic sequ encing on purified cells isolated from human testis.