Z. Varga et al., Inhibition of the superoxide anion release and hydrogen peroxide formationin PMNLs by flavonolignans, PHYTOTHER R, 15(7), 2001, pp. 608-612
The mixture of flavonolignans [Legalon(R) : silybin (2a), isosilybin (3), s
ilydianin (4) and silychristin (5)] and derivatives of silybin (2b-d) were
assessed for their inhibitory activity on the oxidative burst of PMA-stimul
ated human PMNLs. The inhibitory effect of flavonolignans on O-2(-) release
were compared with that of vitamin E (1). The flavonolignans tested exhibi
ted the following order in inhibition Of O-2(-) release by PMA-stimulated P
MNLs: 5,7,4 "- trimethylsilybin (2c) vitamin E (1) > Legalon(R) > peracetyl
silybin (2b) > silybin (2a) > peracetyl-5,7,4 " -trimethylsilybin (2d). The
flavonolignans inhibited not only the O-2(-) release, but also the H2O2 fo
rmation in PMA-stimulated PMNLs. The inhibitory capacity of flavonolignans
on H2O2 formation was similar to their inhibitory capacity on O-2(-) releas
e. These data suggest that the flavonolignans have antioxidant properties o
n the PMNL oxidative burst. The fact that the trimethyl derivative of silyb
in (2c) has a greater inhibitory effect than silybin itself suggests that t
he efficacy of the antioxidant properties is dependent on the lipophilicity
of the molecules. This is underlined by the fact that peracetylation of al
l of the hydroxyl groups in silybin resulted in a total loss of the antioxi
dant activity of the molecule. In summary, flavonolignans inhibit the oxida
tive burst of PMNLs, and this inhibitory effect depends on the chemical str
ucture of the flavonolignans. Copyright (C) 2001 John Wiley & Sons, Ltd.