Isolation of spectrin subunits by reverse-phase high-performance liquid chromatography

Authors
Kakhniashvili, DG Goodman, SR
Citation
Dg. Kakhniashvili et Sr. Goodman, Isolation of spectrin subunits by reverse-phase high-performance liquid chromatography, PROT EX PUR, 23(2), 2001, pp. 249-251
Citations number
12
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PROTEIN EXPRESSION AND PURIFICATION
ISSN journal
10465928 → ACNP
Volume
23
Issue
2
Year of publication
2001
Pages
249 - 251
Database
ISI
SICI code
1046-5928(200111)23:2<249:IOSSBR>2.0.ZU;2-0
Abstract
We present a one-step uncomplicated method of separation of spectrin subuni ts. The method is based on reverse-phase HPLC employing an analytical C4 co lumn. Reverse-phase HPLC combines the steps of dissociation and separation of spectrin subunits. The method can be applied to different spectrin isofo rms. It can be used for analytical purposes, as well as for small-scale (<0 .4 mg) isolation of spectrin subunits. (C) 2001 Academic Press.