A. De Crouy-chanel et G. Richarme, Amount and redox state of cytoplasmic, membrane and periplasmic proteins in Escherichia coli redox mutants, RES MICROB, 152(7), 2001, pp. 663-669
We analyzed the amount and redox state of cytoplasmic, membrane and peripla
smic proteins in Escherichia coli mutants deficient in thioredoxin, thiored
oxin reductase, glutathione and DsbA, by observing the electrophoretic prof
ile of bacterial extracts after in vivo labelling with monobromobimane. Our
results show that these mutations affected not only the amount and the red
ox state of proteins localized in the same compartment as the deficient oxi
doreductase. but also those of the proteins localized in other compartments
. These results concord with the hypothesis that there is a link between th
e redox reactions that occur in the cytoplasm and the periplasm. (C) 2001 E
ditions scientifiques et medicales Elsevier SAS.