T. Hoshi et al., Selective permeation of hydrogen peroxide through polyelectrolyte multilayer films and its use for amperometric biosensors, ANALYT CHEM, 73(21), 2001, pp. 5310-5315
A platinum electrode was coated with polyelectrolyte multilayer (PEM) films
to prepare an amperometric hydrogen peroxide sensor which can be used in t
he presence of possible interferences such as ascorbic acid, uric acid, and
acetaminophen. The PEM films were prepared on the surface of a Pt disk ele
ctrode by an alternate deposition of polycation and polyanion from the aque
ous solutions through electrostatic force of attraction. The Pt electrodes
coated with a poly(allylamine)/poly(vinyl sulfate) or poly(allylamine)/poly
(styrenesulfonate) film were used successfully for detecting H2O2 selective
ly in the presence of the possible interfering agents. It was suggested tha
t H2O2 can diffuse into the PEM film smoothly while the ascorbic acid, uric
acid, and acetaminophen cannot penetrate the film by a size exclusion mech
anism. On the other hand, the electrodes coated with PEM films containing p
oly(ethyleneimine) or poly(diallyl-dimethylammonium chloride) were not usef
ul for the selective determination of H2O2. The results were rationalized b
ased on the different permeability of the films due to the different molecu
lar density or packing in the PEM films. The PEM film-coated electrode was
useful for constructing glucose biosensors by coupling with glucose oxidase
.