Optical determination of low-level water concentrations in organic solvents using fluorescent acridinyl dyes and dye-immobilized polymer membranes

The fluorescent acridinyl indicators 4-(9-acridinyl)-N-(5-hexenyl)-N-methyl aniline (KD-F0011), 6-(9-acridinyl)-,2,2,3-tetramethyl-2,3-dihydro-1H-perim idine (KD-F0021 1), and 6-(9-acridinyl)-2-(3-butenyl)-1,2,3-trimethyl-2,3-d ihydro-1H-perimidine (KD-F0022) were designed, synthesized, and applied for highly sensitive optical determination of low-level water in organic solve nts. All these dyes were found useful as fluorescence indicators for the de tection of water below 1% (v/v) in different solvent media with a low detec tion limit of 0.002% (v/v) or 20 mg/L (22 ppm by weight) for KD-F0021 in TH F solution. Sensing membranes made from poly(ethylene glycol) dimethacrylat e by photocopolymerization with the indicator KD-F0011 were also prepared. Using the membrane sensor, the lowest detection limit of 0.001% (v/v) or 14 mg/L (20 ppm) water was achieved in diethyl ether samples. This system ena bles the continuous monitoring of the water content in a flow-through arran gement, where single-wavelength excitation (404 nm) and single-wavelength d etection (532 nm) can be used for the fluorescence determination, allowing a simple measurement setup. In a continuous-flow experiment using THF sampl es, fully reversible and fast signal changes with t(95%) = 1-2 min for wate r concentrations up to 0.50% (v/v) were observed. A detection limit of 0.00 4% (v/v) or 40 mg/L (45 ppm) water in THF was achieved. These characteristi cs make this type of sensor a useful tool for the online continuous monitor ing of water present as an impurity in organic media, which is difficult to achieve using a Karl Fischer instrument.