Production and partial characterization of mouse monoclonal antibodies recognizing common cytokine receptor gamma chain (gamma(c)) of human, mouse and primate origin

Monoclonal antibodies specific for the common cytokine receptor gamma chain , gamma (c), were produced using traditional hybridoma technology. Fusion o f P3X63-Ag8.653 myeloma cells with splenocytes from Balb/c mice immunized w ith Spodoptera frugiperda insect cells infected with the recombinant baculo virus VL1392-hIL-2R gamma resulted in several hybridoma cell clones produci ng monoclonal gamma (c)-specific antibodies. Four of these antibody-produci ng clones, IIIC3, IIIE8, IG3 and IF10C5, were further characterized by immu noblotting, flow cytometry and ELISA. Data are presented demonstrating that the generated monoclonal antibodies can identify the extracellular domain of the common cytokine receptor gamma chain of human and mouse origin, and two of the antibodies recognize gamma (c) of primate origin as well.