Interrelationships among biological activity, disulfide bonds, secondary structure, and metal ion binding for a chemically synthesized 34-amino-acid peptide derived from alpha-fetoprotein

A 34-amino-acid peptide has been chemically synthesized based on a sequence from human ct-fetoprotein. The purified peptide is active in anti-growth a ssays when freshly prepared in pH 7.4 buffer at 0.20 g/l, but this peptide slowly becomes inactive. This functional change is proven by mass spectrome try to be triggered by the formation of an intrapeptide disulfide bond betw een the two cysteine residues on the peptide. Interpeptide cross-linking do es not occur. The active and inactive forms of the peptide have almost iden tical secondary structures as shown by circular dichroism (CD). Zinc ions b ind to the active peptide and completely prevents formation of the inactive form. Cobalt(II) ions also bind to the peptide, and the UV-Vis absorption spectrum of the cobalt-peptide complex shows that: (1) a near-UV sulfur-to- metal-ion charge-transfer band had a molar extinction coefficient consisten t with two thiolate bonds to Co(II); (2) the lowest-energy visible d-d tran sition maximum at 659 nm, also, demonstrated that the two cysteine residues are ligands for the metal ion; (3) the d-d molar extinction coefficient sh owed that the metal ion-ligand complex was in a distorted tetrahedral symme try. The peptide has two cysteines, and it is speculated that the other two metal ion ligands might be the two histidines. The Zn(II)- and Co(II)-pept ide complexes had similar peptide conformations as indicated by their ultra violet CID spectra, which differed very slightly from that of the free pept ide. Surprisingly, the cobalt ions acted in the reverse of the zinc ions in that, instead of stabilizing anti-growth form of the peptide, they catalyz ed its loss. Metal ion control of peptide function is a saliently interesti ng concept. Calcium ions, in the conditions studied, apparently do not bind to the peptide. Trifluoroethanol and temperature (60 degreesC) affected th e secondary structure of the peptide, and the peptide was found capable of assuming various conformations in solution. This conformational flexibility may possibly be related to the biological activity of the peptide. (C) 200 1 Elsevier Science BN. All rights reserved.