Secretion of Escherichia coli DsbA and DsbC proteins from Brevibacillus choshinensis: stimulation of human epidermal growth factor production

Authors
Tanaka, R Hashimoto, M Ishibashi, M Tokunaga, H Taura, S Mizukami, M Miyauchi, A Tokunaga, M
Citation
R. Tanaka et al., Secretion of Escherichia coli DsbA and DsbC proteins from Brevibacillus choshinensis: stimulation of human epidermal growth factor production, BIOTECH LET, 23(22), 2001, pp. 1853-1857
Citations number
15
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
22
Year of publication
2001
Pages
1853 - 1857
Database
ISI
SICI code
0141-5492(2001)23:22<1853:SOECDA>2.0.ZU;2-K
Abstract
DsbA and DsbC, members of the thioredoxin super-family of redox proteins, w hich are expressed in the periplasmic space of Escherichia coli, were clone d into and successfully secreted from Brevibacillus choshinensis at similar to 100 mug ml(-1). Both proteins were active in exchanging disulfide bonds of bovine insulin in vitro. Furthermore, DsbA secreted by B. choshinensis promoted the conversion of non-native human epidermal growth factor to the native form.