A novel mechanism for myocardial stunning involving impaired Ca2+ handling

The mechanism of myocardial stunning has been studied extensively in rodent s and is thought to involve a decrease in Ca2+ responsiveness of the myofil aments, degradation of Troponin I (TnI), and no change in Ca2+ handling. We studied the mechanism of stunning in isolated myocytes from chronically in strumented pigs. Myocytes were isolated from the ischemic (stunned) and non ischemic (normal) regions after 90-minute coronary stenosis followed by 60- minute reperfusion. Baseline myocyte contraction was reduced, P <0.01, in s tunned myocytes (6.3 +/-0.4%) compared with normal myocytes (8.8 +/-0.4%). The time for 70% relaxation was prolonged, P <0.01. in stunned myocytes (13 1 +/-8 ms) compared with normal myocytes (105 +/-5 ms). The impaired contra ctile function was associated with decreased Ca2+ transients (stunned, 0.33 +/-0.04 versus normal, 0.49 +/-0.05, P <0.01). Action potential measuremen ts in stunned myocytes demonstrated a decrease in plateau potential without a change in resting membrane potential. These changes were associated with decreased L-type Ca2+-current density (stunned, -4.8 +/-0.4 versus normal. -6.6 +/-0.4 pA/pF, P <0.01). There were no differences in TnI, sarcoplasmi c reticulum Ca2+ ATPase (SERCA2a), and phospholamban protein quantities. Ho wever, the fraction of phosphorylated phospholamban monomer was reduced in stunned myocardium. In rats, stunned myocytes demonstrated reduced systolic contraction but actually accelerated relaxation and no change in Ca2+ tran sients. Thus, mechanisms of stunning in the pig are radically different fro m the widely held concepts derived from studies in rodents and involve impa ired Ca2+ handling and dephosphorylation of phospholamban, but not TnI degr adation.