An electrogenerated chemiluminescence imaging fiber electrode chemical sensor for NADH

An electrogenerated chemi luminescence (ECL)-based imaging fiber electrode chemical sensor (IFECS) for the detection of NADH is presented. In brief, a n imaging fiber's distal tip was metalized with gold to serve as an electro de (i,e., an IFE). The 350-mum diameter IFE was coated with a thin, planar layer of Nafion doped with Ru(bpy)(3)(2+) to create an IFECS. An electrical contact was made to the gold layer such that Ru(bpy)(3)(2+) could be oxidi zed to Ru(bpy)3 and electrochemically regenerated following the application of appropriate potentials, Following the diffusion of triethylamine or NAD H into the IFE sensing layer and reaction with Ru(bpy)(3)(3+) the ECL was c aptured using a charge coupled device-based imaging system. The triethylami ne calibration plot was linear between 0.0 and 50 mM. IFECSs represent the first demonstration whereby ECL imaging is performed through the actual sen sor itself.