De. Cohn et al., Genotypic and phenotypic progression in endometrial tumorigenesis: Determining when defects in DNA mismatch repair and KRAS2 occur, GENE CHROM, 32(4), 2001, pp. 295-301
We set out to determine the relative timing of loss of DNA mismatch repair
and KRAS2 mutation in endometrial tumorigenesis. We studied endometrial car
cinoma (CA) and synchronous atypical endometrial hyperplasia (AEH), the pre
malignant precursor of endometrial cancer. Carcinoma and hyperplasia were i
nvestigated for loss of mismatch repair as evidenced by microsatellite inst
ability (MSI) and for KRAS2 mutations. Endometrial cancers previously shown
to be MSI-positive were evaluated for KRAS2 codon 12 and 13 mutations. DNA
was isolated from foci of AEH concomitant with, but physically remote from
, the cancers by use of tissues prepared by laser capture microdissection (
LCM). The AEH DNAs were then assessed for MSI and KRAS2 mutations. Of 210 e
ndometrial CAs investigated, 51 (26%) were MSI-positive, and among those, 2
1 (41%) arose concomitantly with AEH. Of 41 foci of AEH (mean, two foci per
patient) investigated, 34 (83%) were MSI-positive. KRAS2 mutations were se
en in 5/51 (10%) MSI-positive carcinomas. From the five patients informativ
e for both KRAS2 mutation and MSI, 10 foci of AEH were available for invest
igation. All 10 AEH specimens (100%) were MSI-positive, and six (60%) had t
he KRAS2 mutation present in the coexisting CA. The observation that some M
SI-positive AEH specimens lack the KRAS2 mutation seen in the coexisting CA
supports a model in which loss of DNA mismatch repair precedes KRAS2 mutat
ion. However, in addition to the absence of KRAS2 mutations in AEH, we disc
overed mutations in LCM hyperplasia and carcinoma specimens that were not p
resent in the portion of the cancers originally investigated. These discord
ant genotypes suggest genetic heterogeneity in endometrial hyperplasia and
concomitant cancer. (C) 2001 Wiley-Liss, Inc.