High-density marker loss of heterozygosity analysis of rat chromosome 10 in endometrial adenocarcinoma

Endometrial cancer is a disease with serious impact on the human population , but not much is known about genetic factors involved in this complex dise ase. Female BDII rats are genetically predisposed to spontaneous endometria l carcinoma, and the BDII inbred strain provides an experimental animal mod el for endometrial carcinoma development. In the present study, BDII female s were crossed with males from two nonsusceptible inbred rat strains. Endom etrial adenocarcinomas (EACs) developed in a proportion of the F1 and F2 pr ogeny. We screened 18 EAC solid tumors and 9 EAC cell cultures for loss of heterozygosity (LOH) using fluorescent-PCR-based marker allelotyping method ology with 47 microsatellite markers covering the proximal part of rat chro mosome 10 (RNO 10). Conclusive evidence was obtained for LOH/deletion invol ving about 56 cM in the proximal part of RNO 10 in DNA from six out of seve n informative tumor cell cultures. Analysis of the solid tumors confirmed t he presence of LOH in this part of RNO 10 in 14 of 17 informative tumors. H owever, from the studies in the solid tumors it appeared that in fact three separate segments in the proximal part of RNO 10 were affected. These thre e LOH/deletion regions were located approximately in cytogenetic bands 10q1 1-12, 10q22, and 10q24. (C) 2001 Wiley-Liss, Inc.