A. Behboudi et al., High-density marker loss of heterozygosity analysis of rat chromosome 10 in endometrial adenocarcinoma, GENE CHROM, 32(4), 2001, pp. 330-341
Endometrial cancer is a disease with serious impact on the human population
, but not much is known about genetic factors involved in this complex dise
ase. Female BDII rats are genetically predisposed to spontaneous endometria
l carcinoma, and the BDII inbred strain provides an experimental animal mod
el for endometrial carcinoma development. In the present study, BDII female
s were crossed with males from two nonsusceptible inbred rat strains. Endom
etrial adenocarcinomas (EACs) developed in a proportion of the F1 and F2 pr
ogeny. We screened 18 EAC solid tumors and 9 EAC cell cultures for loss of
heterozygosity (LOH) using fluorescent-PCR-based marker allelotyping method
ology with 47 microsatellite markers covering the proximal part of rat chro
mosome 10 (RNO 10). Conclusive evidence was obtained for LOH/deletion invol
ving about 56 cM in the proximal part of RNO 10 in DNA from six out of seve
n informative tumor cell cultures. Analysis of the solid tumors confirmed t
he presence of LOH in this part of RNO 10 in 14 of 17 informative tumors. H
owever, from the studies in the solid tumors it appeared that in fact three
separate segments in the proximal part of RNO 10 were affected. These thre
e LOH/deletion regions were located approximately in cytogenetic bands 10q1
1-12, 10q22, and 10q24. (C) 2001 Wiley-Liss, Inc.