Cancer of the uterine cervix may be significantly associated with a gene polymorphism coding for increased IL-10 production

The purpose of our prospective, case-controlled study was to investigate th e hypothesis that women who are genetically programmed to produce high or m edium levels of IL-10 were more likely to develop cancer of the uterine cer vix than individuals genetically predisposed to low IL-10 production. The p opulation was recruited from patients attending gynecological clinics at 2 hospitals in Harare, Zimbabwe. Laboratory tests were performed in the Depar tments of Immunology, Chemical Pathology and Medical Microbiology, Medical School, University of Zimbabwe, and simultaneously at the Department of Bio logical Sciences, University of Manchester, United Kingdom. Included in our study were 77 women with histologically proven cancer of the uterine cervi x and 69 age- and parity-matched healthy women. All of the patients and hea lthy controls were from the Shona ethnic group that inhabits northern Zimba bwe. DNA was purified from cervical cytobrush samples obtained from women w ith cervical cancer. Control DNA was extracted from urine or peripheral blo od samples from the healthy women. The Qiagen DNA extraction kit was used. Detection of allele A and/or G at -1082 in the promoter region of the IL-10 gene was carried out using the ARMS-PCR technique. Polymorphism in the amp lified products was detected by gel electrophoresis in the presence of ethi dium bromide and were bands visualized under UV light. The data comprise 77 women who developed invasive cervical cancer and 69 healthy women matched for age and parity. Patients with cancer were significantly (p = 0.001) mor e likely to be predisposed to produce higher (A/G) levels of IL-10. The gen otype encoding for high (GIG) production of IL-10 was only observed in one cancer patient. The prevalence of low producers of IL-10 in the cancer grou p was significantly lower than in the healthy women. There were no high pro ducers amongst the healthy women. These data suggest that the genetically a cquired ability to produce higher levels of IL-10 may be a significant fact or in the development of cervical cancer. (C) 2001 Wiley-Liss, Inc.