Influence of surface chemistry on particle-particle aggregation as measured by a Coulter Counter in the low concentration regime

Citation
S. Trenor et al., Influence of surface chemistry on particle-particle aggregation as measured by a Coulter Counter in the low concentration regime, J ADHES SCI, 15(10), 2001, pp. 1189-1198
Citations number
12
Categorie Soggetti
Material Science & Engineering
Journal title
JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY
ISSN journal
01694243 → ACNP
Volume
15
Issue
10
Year of publication
2001
Pages
1189 - 1198
Database
ISI
SICI code
0169-4243(2001)15:10<1189:IOSCOP>2.0.ZU;2-R
Abstract
The link between particle concentration, surface properties, and particle a ggregation has been investigated for polystyrene latex in the low particle concentration regime, volume fractions less than 1 x 10(-6). The particles used in this model study were 9.14-mum diameter polystyrene beads, approxim ately the same size as red blood cells, using a Coulter Counter with a 100- mum aperture as a gauge of clustering. The lower concentrations of beads di splayed the same trend as reported in other publications. Albumin and fibrinogen were then attached to the beads and the effect of no n-specific binding by the proteins on particle agglomeration was studied. A lbumin showed little effect on the number of aggregates encountered whereas fibrinogen increased them number of aggregates, indicating that stronger p article interactions occurred between the fibrinogen coated particles. The addition of stearic acid had no marked effect on the aggregation properties of the beads, coated or uncoated. The addition of sodium stearate, however , increased the number of doublets, triplets, and multiplets formed. These results may be relevant to adhesion of cells. Our approach is a plausible w ay to consider the roles that proteins and fatty acid molecules have on the relative binding potential without the constraints of using live cells.