S. Trenor et al., Influence of surface chemistry on particle-particle aggregation as measured by a Coulter Counter in the low concentration regime, J ADHES SCI, 15(10), 2001, pp. 1189-1198
The link between particle concentration, surface properties, and particle a
ggregation has been investigated for polystyrene latex in the low particle
concentration regime, volume fractions less than 1 x 10(-6). The particles
used in this model study were 9.14-mum diameter polystyrene beads, approxim
ately the same size as red blood cells, using a Coulter Counter with a 100-
mum aperture as a gauge of clustering. The lower concentrations of beads di
splayed the same trend as reported in other publications.
Albumin and fibrinogen were then attached to the beads and the effect of no
n-specific binding by the proteins on particle agglomeration was studied. A
lbumin showed little effect on the number of aggregates encountered whereas
fibrinogen increased them number of aggregates, indicating that stronger p
article interactions occurred between the fibrinogen coated particles. The
addition of stearic acid had no marked effect on the aggregation properties
of the beads, coated or uncoated. The addition of sodium stearate, however
, increased the number of doublets, triplets, and multiplets formed. These
results may be relevant to adhesion of cells. Our approach is a plausible w
ay to consider the roles that proteins and fatty acid molecules have on the
relative binding potential without the constraints of using live cells.