Repeated cocaine administration attenuates group I metabotropic glutamate receptor-mediated glutamate release and behavioral activation: A potential role for Homer
Cj. Swanson et al., Repeated cocaine administration attenuates group I metabotropic glutamate receptor-mediated glutamate release and behavioral activation: A potential role for Homer, J NEUROSC, 21(22), 2001, pp. 9043-9052
The present study aimed to characterize a functional role for group I metab
otropic glutamate receptors (mGluRs) in the nucleus accumbens and the capac
ity of repeated cocaine to elicit long-term changes in group I mGluR functi
on. Reverse dialysis of the group I agonist (RS)-3,5-dihydroxyphenylglycine
(DHPG) into the nucleus accumbens resulted in an increase in extracellular
glutamate levels that was mediated by the mGluR1 subtype and depended on v
oltage-dependent Na+ and Ca2+ conductance. At 3 weeks after discontinuing 1
week of daily cocaine injections, the capacity of DHPG to induce glutamate
release was markedly reduced. Similarly, DHPG induced an mGluR1-dependent
increase in locomotor activity after microinjection into the nucleus accumb
ens that was significantly blunted 3 weeks after repeated cocaine administr
ation. Signaling through group I mGluRs is regulated, in part, by Homer pro
teins, and it was found that the blunting of group I mGluR-induced glutamat
e release and motor activity after repeated cocaine was associated with a r
eduction in Homer1b/c protein that was selective for the medial nucleus acc
umbens. These data show that repeated cocaine produces an enduring inhibiti
on of the neurochemical and behavioral consequences of stimulating mGluR1 t
hat is accompanied by changes in the mGluR scaffolding apparatus.