Experimental glaucoma in the primate induced by latex microspheres

The injection of sterile latex microspheres into the anterior chamber of th e eye is presented as a simple and cost effective method for inducing chron ic elevation of intraocular pressure (IOP) and experimental glaucoma in pri mates. The microspheres produce elevated IOP primarily by restricting the o utflow of aqueous humor through the trabecular meshwork located in the cham ber angle. Different levels and durations of elevated IOP can be obtained b y altering the frequency and number of microspheres injected. In comparison with other primate models of experimental glaucoma, the approach described here has the advantages of producing chronic elevations of IOP without the need for expensive ophthalmic equipment and personnel, surgical interventi on or intraocular inflammation, and without compromising visibility of the optic disc, which is necessary for clinical assessment of the onset and pro gression of the disease. (C) 2001 Elsevier Science B.V. All rights reserved .