Vaccination with attenuated simian immunodeficiency virus by DNA inoculation

Authors
Kent, SJ Dale, CJ Preiss, S Mills, J Campagna, D Purcell, DFJ
Citation
Sj. Kent et al., Vaccination with attenuated simian immunodeficiency virus by DNA inoculation, J VIROLOGY, 75(23), 2001, pp. 11930-11934
Citations number
21
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
75
Issue
23
Year of publication
2001
Pages
11930 - 11934
Database
ISI
SICI code
0022-538X(200112)75:23<11930:VWASIV>2.0.ZU;2-K
Abstract
Delivering attenuated lentivirus vaccines as proviral DNA would be simple a nd inexpensive. Inoculation of macaques with wild-type simian immunodeficie ncy virus strain mac239 (SIVmac239) DNA or SIVmac239 DNA containing a singl e deletion in the 3' nef-long terminal repeat overlap region (nef/LTR) led to sustained SIV infections and AIDS. Injection of SIVmac239 DNA containing identical deletions in both the 5' LTR and 3' nef/LTR resulted in attenuat ed SIV infections and substantial protection against subsequent mucosal SIV mac251 challenge.