We investigated the conditions promoting toluene biodegradation for gasolin
e-contaminated nearsurface (0.6 m depth) and subsurface (4.7 to 5.0 m depth
) vadose zone soils sampled from an and environment. At both depths, water
addition was required for toluene biodegradation to occur. In near-surface
samples, no inorganic nutrient addition was necessary and (i) biodegradatio
n was fastest at 0.0 MPa, (ii) biodegradation rates decreased with decreasi
ng water potential down to -1.0 MPa, and (iii) biodegradation was undetecta
ble at -1.5 MPa. For subsurface material, toluene depletion was stimulated
either by slurrying with a nutrient solution or by adjusting the moisture c
ontent to 20% (0.0 MPa) with nutrient solution and lowering the oxygen conc
entration (to effectively 1 mg L-1 in the aqueous phase). Thus, in the subs
urface material, toluene depletion was microaerobic and nutrient-limited, o
ccurring only under low oxygen and with inorganic nutrient addition. Our st
udies implicate microaerophily as an important characteristic of the toluen
e-degrading communities in these dry soils, with soil water as a primary co
ntroller of oxygen availability.