Community dynamics of free-living and particle-associated bacterial assemblages during a freshwater phytoplankton bloom

Bacterial community dynamics were followed in a 19-day period during an ind uced diatom bloom in two freshwater mesocosms, The main goal was to compare diversity and succession among free-living (< 10 mum) and particle-associa ted (> 10 mum) bacteria. Denaturing gradient gel electrophoresis (DGGE) of PCR amplified 16S rDNA showed the highest number of bands among free-living bacteria, but with a significant phylogenetic overlap in the two size frac tions indicating that free-living bacteria were also important members of t he particle-associated bacterial assemblage. Whereas the number of bands in the free-living fraction decreased during the course of the bloom, several phylotypes unique to particles appeared towards the end of the experiment. Besides the primer set targeting Bacteria, a primer set targeting most mem bers of the Cytophaga-Flavobacterium (CF)-cluster of the Cytophaga-Flavobac terium-Bacteroides group and a primer set mainly targeting alpha -Proteobac teria were applied. PCR-DGGE analyses revealed that a number of phylotypes targeted by those primer sets were found solely on particles. Almost all se quenced bands from the bacterial DGGE gel were related to phylogenetic grou ps commonly found in freshwater: alpha -Proteobacteria, CF, and Firmicutes. Despite the use of primers intended to be specific mainly for alpha -Prote obacteria most bands sequenced from the alpha -proteobacterial DGGE gel for med a cluster within the Verruco-microbiales subdivision of the Verrucomicr obia division and were not related to alpha -Proteobacteria. Bands sequence d from the CF DGGE gel were related to members of the CF cluster. From the present study, we suggest that free-living and particle-associated bacteria l communities should not be perceived as separate entities, but rather as i nteracting assemblages, where the extent of phylogenetic overlap is depende nt on the nature of the particulate matter.