Diversity of the carbohydrate composition of the antigenic polysaccharidesof proteobacteria of the genera Pseudoalteromonas and Marinomonas

The sugar analysis of the glycans of the type strains of marine proteobacte ria of the genera Pseudoalteromonas and Marinomonas-Pseudoaltetromonas atla ntica IAM12927(T), P. aurantia NCIMB 2033(T), P. citrea ATCC 29719(T), P. e lyakovii KMM 162(T), P. espejiana ATCC 29659(T), P. piscicida NCIMB 645(T), P tetraodonis JAM 14160(T), Marinomonas communis ATCC 27118(T) and M. vaga ATCC 27119(T) - showed that they contain glucose, galactose, galactosamine , glucosamine, fucose, rhamnose, mannose, heptose, 2-keto-3-deoxyoctonate ( KDO), uronic acids, colitose (3,6-dideoxy-L-xylo-hexose), and 6-deoxy-L-tal ose. The carbohydrate composition of the antigenic polysaccharides (PSs) of P elyakovii KMM 162(T) and P espejiana ATCC 29659(T) depended on the type and the concentration of carbohydrate substrates in the nutrient media. The molar proportion between rhamnose, glucose, and galactose (ca. 1 : 0.3 : 2 ) in the PS of P. elyakovii KMM 162(T) was almost the same in the media lac king carbohydrates or containing glucose or galactose at a concentration of 1 g/l. At the same time, the molar proportion between fucose, glucose, gal actose, galactosamine, and glucosamine (ca. 1 : 1 : 1 : 2 : 0.5) in the PS of R espejiana ATCC 29659(T) depended on the presence and the concentration of carbohydrate substrates in the medium. A high concentration of glucose in the medium (30 g/l) brought about a rise in the content of glucose in PS s (9-fold for the PS of P. elyakovii KMM 162(T) and 4.6-fold for the PS of P espejiana ATCC 29659(T)) and led to a decrease in the content of other ca rbohydrates. The cultivation of these two strains at a lactose concentratio n of 30 all resulted in their PSs containing glucose and galactose in about equal proportions (ca. 1 : 1 in the case of P. espejiana ATCC 29659(T) and ca. 2.1 : 1.7 in the case of P. elyakovii KMM 162(T)).