Brain-specific sequence Hfb1 is a part of the large 3 '-untranslated region of the human complexin 2 gene: New in vitro and in silico data

A genomic clone hybridizing with brain-specific sequence Hfb1 was isolated from a chromosome 5 cosmid library. Hfb1 proved to correspond to a new gene exon which codes for a large 3'-untranslated region of the mRNA for synapt ic protein complexin 2. Together with the 985-nt Hfb1 cDNA (EMBLY15167) iso lated previously from a cDNA library of the frontal cerebral cortex, the pr imary structure was established for genomic clone Ghfb sized more than 4 kb . A GenBank search revealed complete identity of the 5' end of Ghfb and the 3'-untranslated region (878-933) of the human complexin 2 mRNA. Large tran scripts with the 5' end corresponding to the complexin 2 mRNA and the 3' en d to Ghfb were detected in total mRNA of the human brain by means of RT-PCR . The size of the 3'-untranslated region of the human complexin 2 mRNA was estimated at 4 kb.