The effect of azidothymidine on reactivation of DNA synthesis in macrophage nuclei contained in heterokaryons

Authors
Kazimirchuk, EV Yegorov, YE Karachentsev, DN Zelenin, AV
Citation
Ev. Kazimirchuk et al., The effect of azidothymidine on reactivation of DNA synthesis in macrophage nuclei contained in heterokaryons, MOL BIOL, 35(5), 2001, pp. 775-777
Citations number
18
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
00268933 → ACNP
Volume
35
Issue
5
Year of publication
2001
Pages
775 - 777
Database
ISI
SICI code
0026-8933(200109/10)35:5<775:TEOAOR>2.0.ZU;2-6
Abstract
In heterokaryons, DNA synthesis is reactivated in macrophage nuclei only in the case of fusion with immortal cells. Assuming that telomerase is respon sible for reactivation, the effect of its inhibitor azidothymidine (AZT) wa s studied in heterokaryons of mouse resident peritoneal macrophages and imm ortal 3T3 Swiss cells. AZT suppressed reactivation of DNA synthesis in macr ophage nuclei and had no effect on DNA synthesis in 3T3 Swiss cell nuclei, suggesting that telomere structure is impaired in normal mouse macrophages.