Physiological concentrations of genistein stimulate the proliferation and protect against free radical-induced oxidative damage of MC3T3-E1 osteoblast-like cells
Ys. Lee et al., Physiological concentrations of genistein stimulate the proliferation and protect against free radical-induced oxidative damage of MC3T3-E1 osteoblast-like cells, NUTR RES, 21(9), 2001, pp. 1287-1298
Genistein, a phytoestrogen and an antioxidant, acts on osteoblast-like cell
s by increasing cellular proliferation. The present, study was designed to
determine the antioxidative action of genistein on the viability of MC3T3-E
1 cells under oxidative stress induced by free radicals during cell prolife
ration. After incubation for 24 hr, cells were treated with oxidative catal
ysts, H2O2 and FeSO4, and the isoflavones (genistein and daidzein) for anot
her 6 hr. Also, cells were treated with genistein or antioxidants (vitamin
E or C) with or without H2O2 and FeSO4. The viability of cells treated with
free radicals was significantly decreased (p < 0.05), whereas treatment wi
th genistein at physiological concentrations reduced the damage to cells by
free radicals over 6 hr (p < 0.05), and was more effective than either vit
amin E or C. These results suggest that genistein not only stimulates cell
proliferation but also protects against oxidative cellular damage of free r
adicals in osteoblast-like cells. (C) 2001 Elsevier Science Inc. All rights
reserved.