DETECTION AND LOCALIZATION OF GONADOTROPIN-RELEASING-HORMONE (GNRH)-LIKE MATERIAL IN THE FROG, RANA-ESCULENTA, OVARY

GnRH-like material has been identified using HPLC followed by RIA in t he ovary of Rana esculenta. During the reproductive cycle three immuno reactive GnRH peaks were eluted. One of them coeluted with s-GnRH, the other two forms between GnRH and cII-GnRH. During the recovery phase s-GnRH immunoreactivity disappears. By immunocytochemistry, cII-GnRH i mmunostaining was localized to granulosa cells while s-GnRH was presen t in the perinuclear zone of the oocytes.