In this study, semen samples from sixty bulls were used for virus isolation
into sensitive cell culture. Five viruses were isolated by inoculation of
60 semen samples into the cell cultures. The isolated viruses were identifi
ed as BHV-1 according to results of cross microneutralization test by using
hyperimmun sera from rabbits. Obtained data showed that BHV-1 may exist in
the semen, and sensitive cattle may be infected by virus via insemination.