A calmodulin-dependent protein kinase (MCK1) appeared important in regulati
ng flowering in tobacco. The expression of modified MCK1 that lacks the C-t
erminal including calmodulin-binding domain upsets the flowering developmen
tal program, leading to the abortion of flower primordia initiated on the m
ain axis of the plant and, as well, caused the prolongation of the vegetati
ve phase in axillary buds. The abortion process of flowers began first in t
he developing anthers and subsequently the entire flower senesces. In axill
ary buds the prolonged vegetative phase was characterized by atypical elong
ated, narrow, twisted leaves. These results suggested a role for calmodulin
-dependent protein kinase homologs in mediating flowering.