One hundred and five donkeys were examined over a 12-month period for the p
resence of Gasterophilus spp. larvae in their stomachs. G. intestinalis and
G. nasalis were found, but there was a distinct seasonal variation in thei
r abundance. The numbers of second-stage larvae (L-2) peaked during the ear
ly winter and early summer, but they were absent or at low levels during th
e early spring and early autumn. This suggests that there are two periods o
f adult fly activity (late autumn and late spring) for both G. nasalis and
G. intestinalis. Third-stage larvae (L-3) were found throughout the year, b
ut the lowest levels were seen in the late summer and autumn. The parasites
also had an aggregated distribution, which may be due to host selection by
the fly. The numbers of larvae did not vary significantly with age, which
may be due to a lack of acquired protective immunity or to tolerance after
repeated exposure.