Bk. Singh et al., Neutralizing and complement-fixing monoclonal antibodies as an aid to the diagnosis of equine herpesvirus-1 infection, VET RES COM, 25(8), 2001, pp. 675-686
One complement-fixing (C-MAb) and three complement-dependent neutralizing m
onoclonal antibodies (N-MAbs) were raised against Hisar-90-7 equine herpesv
irus-1 (EHV-1) strain. The target antigen of the C-MAb (2A5) and two of the
N-MAbs (1H6, 9C4) was identified as a 140 kDa polypeptide in Western blott
ing. The target antigen of N-MAb (9C6) could not be identified. Purified po
lypeptides of five EHV-1 strains isolated from different regions and at dif
ferent times gave intense bands at 140 kDa when reacted with N-MAb (1H6) in
Western blots. In sandwich ELISA, all four MAbs captured the viral antigen
from clinical materials, giving a reliable and rapid diagnosis of EHV-1 in
fection in equines.