Whm. Vanderpoel et al., EXPERIMENTAL REPRODUCTION OF RESPIRATORY-DISEASE IN CALVES WITH NON-CELL-CULTURE-PASSAGED BOVINE RESPIRATORY SYNCYTIAL VIRUS, Veterinary quarterly, 18(3), 1996, pp. 81-86
To reproduce experimentally clinical bovine respiratory syncytial viru
s (BRSV) infections in cattle, we isolated BRSV from a calf in the fie
ld that suffered from acute respiratory disease, Cell culture passage
of the virus was avoided to prevent any modification of the biological
properties of the virus. The isolated BRSV was passaged in specific-p
athogen-free (SPF) calves, Lung lavage fluids of these calves, which c
ontained at least 10(3) TCID50/ml BRSV and which were found to be free
of other known respiratory pathogens, were collected and pooled for e
xperimental infection. To reproduce a clinical BRSV infection, two gro
ups of six SPF calves were inoculated intranasally with 2 mi of 10(3.9
) TCID50/ml BRSV of the obtained virus stock. Another five calves, whi
ch were persistently infected with bovine virus diarrhoea virus (BVDV)
, were given the same inoculum, One group of six calves served as mock
-infected controls. Clinical signs were closely monitored from 1 week
before until 16 days after inoculation. Reproducible clinical signs co
nsisting of significantly (p<0.05) increased respiratory rates and ele
vated body temperatures were recorded but not in all BRSV-inoculated c
alves, Although clinical signs were induced by experimental infection
with non-cell-culture-passaged BRSV, the respiratory signs were not as
serious as in the most severe cases in the field.