M. Yang et al., Interleukin-13 mediates airways hyperreactivity through the IL-4 receptor-alpha chain and STAT-6 independently of IL-5 and eotaxin, AM J RESP C, 25(4), 2001, pp. 522-530
Citations number
48
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
Interleukin (IL)-13 is a central mediator of the processes underlying the i
nduction of airways hyperreactivity (AHR) in the allergic lung. However, th
e mechanisms by which IL-13 induces AHR and the associated role of inflamma
tory infiltrates as effector cells has not been fully elucidated. In this i
nvestigation, we show that intratracheal administration of IL-13 induces AH
R in the presence and absence of inflammation. The initial AHR response (pe
ak, 6 to 24 h; preinflammatory phase [PIP]) was dissociated from inflammati
on (eosinophilia) and mucus hypersecretion but was critically regulated by
signaling through the IL-4 receptor a chain (IL-4R alpha) and signal transd
ucers and activators of transcription (STAT)-6. The second response (> 24 h
, inflammatory phase [IP]) was characterized by an amplified AHR, eosinophi
l accumulation, and mucus hypersecretion. These features of the IP were not
observed in IL-4R alpha- or STAT-6-deficient mice. To determine the role o
f eosinophils in the induction of IP AHR and mucus hypersecretion, we admin
istered IL-13 to IL-5-, eotaxin-, and IL-5/eotaxin-deficient mice. IL-13-me
diated eosinophil accumulation was significantly attenuated (but not ablate
d) in IL-5-, eotaxin-, or IL-5/eotaxin-deficient mice. However, IL-13-induc
ed AHR and mucus secretion occurred independently of IL-5 and/or eotaxin. T
hese findings demonstrate that IL-13 can induce AHR independently of these
eosinophil regulatory cytokines and mucus hypersecretion. Furthermore, IL-1
3-induced AHR, eosinophilia, and mucus production are critically dependent
on the IL-4R alpha chain and STAT-6.