Attenuation and immunogenicity in humans of a live dengue virus type-4 vaccine candidate with a 30 nucleotide deletion in its 3 '-untranslated region

Citation
Ap. Durbin et al., Attenuation and immunogenicity in humans of a live dengue virus type-4 vaccine candidate with a 30 nucleotide deletion in its 3 '-untranslated region, AM J TROP M, 65(5), 2001, pp. 405-413
Citations number
43
Categorie Soggetti
Envirnomentale Medicine & Public Health","Medical Research General Topics
Journal title
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
ISSN journal
00029637 → ACNP
Volume
65
Issue
5
Year of publication
2001
Pages
405 - 413
Database
ISI
SICI code
0002-9637(200111)65:5<405:AAIIHO>2.0.ZU;2-M
Abstract
The recombinant dengue virus type-4 vaccine candidate 2A Delta 30 was atten uated in rhesus monkeys due to an engineered 30-nucleotide deletion in the 3'-untranslated region of the viral genome. A clinical trial to evaluate th e safety and immunogenicity of a single dose of 2A Delta 30 was conducted w ith 20 adult human volunteers. The vaccine candidate was well tolerated and did not cause systemic illness in any of the 20 volunteers. Viremia was de tectable in 14 volunteers at a mean level of 1.6 log(10) plaque-forming uni ts/ml of serum, although all 20 volunteers seroconverted with a seven-fold or greater increase in serum neutralizing antibody titer on day 28 post-vac cination (mean titer = 1:580). A mild, asymptomatic, macular rash developed in 10 volunteers, and a transient elevation in the serum level of alanine aminotransferase was noted in five volunteers. The low level of reactogenic ity and high degree of immunogenicity of this vaccine candidate warrant its further evaluation and its use to create chimeric vaccine viruses expressi ng the structural genes of dengue virus types 1, 2, and 3.