3D STRUCTURAL INFORMATION AS A GUIDE TO PROTEIN ENGINEERING USING GENETIC SELECTION

Authors
KAST P HILVERT D
Citation
P. Kast et D. Hilvert, 3D STRUCTURAL INFORMATION AS A GUIDE TO PROTEIN ENGINEERING USING GENETIC SELECTION, Current opinion in structural biology, 7(4), 1997, pp. 470-479
Citations number
80
Categorie Soggetti
Cell Biology",Biology
Journal title
Current opinion in structural biology → ACNP
ISSN journal
0959440X
Volume
7
Issue
4
Year of publication
1997
Pages
470 - 479
Database
ISI
SICI code
0959-440X(1997)7:4<470:3SIAAG>2.0.ZU;2-E
Abstract
A great variety of protein systems have been investigated in the past year using structure-guided evolutionary strategies. On the basis of a vailable 3D structural information, critical regions of proteins have been targeted for randomizing mutagenesis and active variants of the c orresponding genes have been selected. These approaches help character ize structural and mechanistic features of proteins and have important implications far design.