ELECTRON AND ATOMIC-FORCE MICROSCOPY OF MEMBRANE-PROTEINS

Authors
HEYMANN JB MULLER DJ MITSUOKA K ENGEL A
Citation
Jb. Heymann et al., ELECTRON AND ATOMIC-FORCE MICROSCOPY OF MEMBRANE-PROTEINS, Current opinion in structural biology, 7(4), 1997, pp. 543-549
Citations number
66
Categorie Soggetti
Cell Biology",Biology
Journal title
Current opinion in structural biology → ACNP
ISSN journal
0959440X
Volume
7
Issue
4
Year of publication
1997
Pages
543 - 549
Database
ISI
SICI code
0959-440X(1997)7:4<543:EAAMOM>2.0.ZU;2-2
Abstract
Electron crystallography is becoming a powerful tool for the resolutio n of membrane protein structures. The past year has seen the productio n of a bacteriorhodopsin model at 3.5 Angstrom and the structure of aq uaporin 1 approaching atomic resolution. Determination of surface topo graphies of 2D crystals using the atomic force microscope is similarly advancing to a level that reveals submolecular details. As the latter is operated in solution, membrane proteins can be observed at work.