Development and validation of a method for the confirmation of nicarbazin in chicken liver and eggs using LC-electrospray MS-MS according to the revised EU criteria for veterinary drug residue analysis
S. Yakkundi et al., Development and validation of a method for the confirmation of nicarbazin in chicken liver and eggs using LC-electrospray MS-MS according to the revised EU criteria for veterinary drug residue analysis, ANALYST, 126(11), 2001, pp. 1985-1989
A method is described for the quantitative confirmation of 4,4'-dinitrocarb
anilide (DNC), the marker residue for nicarbazin in chicken liver and eggs.
The method is based on LC coupled to negative ion electrospray MS-MS of ti
ssue extracts prepared by liquid-liquid extraction. The [M-H](-) ion at m/z
301 is monitored along with two transition ions at m/z 137 and 107 for DNC
and the [M-H](-) ion at m/z 309 for the internal standard, d(8)-DNC. The m
ethod has been validated according to the new EU criteria for the analysis
of veterinary drug residues at 100, 200 and 300 mug kg(-1) in liver and at
10, 30 and 100 mug kg(-1) in eggs. Difficulties concerning the application
of the new analytical limits, namely the decision limit (CC) and the detect
ion capability (CC) to the determination of DNC in both liver and eggs are
discussed.