X. Zhang et Jv. Sweedler, Ultraviolet native fluorescence detection in capillary electrophoresis using a metal vapor NeCu laser, ANALYT CHEM, 73(22), 2001, pp. 5620-5624
Ultraviolet (UV) excitation for laser-induced native fluorescence (LINF) de
tection in capillary electrophoresis (CE) offers impressive performance fig
ures of merit when assaying peptides containing tyrosine or tryptophan resi
dues, catecholamines, indolamines, and a number of other classes of analyte
s with appreciable fluorescence when excited by UV radiation. One of the la
rgest drawbacks of native fluorescence detection schemes in CE-LINF systems
has been the expense and the complexity of the lasers required for excitat
ion in the deep UV wavelength range of 200-300 nm. An improved "turn-key" N
eCu laser operating at 248.6 nm interfaced to a sheath flow-based CE system
obtains a performance similar to that of large frame frequency-doubled Ar
ion lasers. The detection limits for serotonin and dopamine (27 nM and 8 mu
M, respectively, for similar to3-nL injection) are similar to those obtaine
d using a frequency-doubled Ar ion laser at 257 mn (21 nM and 8 muM, respec
tively). An example of the detection of serotonin-related analytes from a s
ingle-cell electropherogram demonstrates the performance of such a system f
or mass-limited measurements.