IMPAN cells: A pancreatic model for differentiation into endocrine cells

It is currently believed that pancreatic progenitor or stem cells exist in the ductal cell population and that these cells have the ability to be grow n and differentiated into endocrine cells for the treatment of diabetes. In this study, we have examined this potential in IMPAN (Immortalized Pancrea tic) cells. These cells are derived from the adult H-2K(b)-tsA58 transgenic mouse. We observed an increased m-RNA expression of insulin, proendocrine gene neurogenin 3, and beta -cell transcription factor Pdx1 when the cells were grown on bovine collagen I gels. The induction profile of these three genes was similar under the tested conditions. No glucagon or other endocri ne-specific transcription factors were detectable. Application of GIP, GLP- 1 derivative NN2211, and activin-A/betacellulin to IMPAN cells in normal cu lture did not lead to endocrine differentiation. In conclusion, it appears that the ability of IMPAN cells to mature to endocrine cells is limited. (C ) 2001 Academic Press.