Pulsatile flow regulates monocyte adhesion to oxidized lipid-induced endothelial cells

Oxidized 1-palmitoyl-2-aracbidonoyl-sn-glycero-3-phosphorylcholine (ox-PAPC ), a component of minimally modified low density lipoprotein, induces monoc yte adhesion to endothelial cells. It is not known whether the upstroke slo pes of pulsatile flow, defined as shear stress slew rates (partial derivati ve tau/partial derivativet), can regulate monocyte binding to ox-PAPC-treat ed bovine aortic endothelial cells (BAECs). At 60 cycles per minute, ox-PAP C-treated BAECs were exposed to 3 conditions representing known vascular co nditions: (1) high shear stress slew rate (partial derivative tau/partial d erivativet = 293 dyne. cm(-2) (.) s(-1)), with time-averaged shear stress = 50 dyne/cm(2); (2) low shear stress slew rate (partial derivative tau/part ial derivativet = 71 dyne (.) cm(-2 .) s(-1)), with identical time-averaged shear stress; and (3) reversing oscillating flow (0 +/- 2.6 mm Hg). Revers e transcription-polymerase chain reaction and quantification were performed for monocyte chemoattractant protein-1 (MCP-1) mRNA expression. High parti al derivative tau/partial derivativet reduced monocyte binding to ox-PAPC-t reated BAECs by 64 +/- 3.2% compared with static conditions, and low partia l derivative tau/partial derivativet reduced monocyte binding by 31 +/- 3.4 %, whereas oscillating flow increased monocyte binding by 22 +/- 1.7% (P < 0.005). High partial derivative tau/partial derivativet downregulated MCP-1 expression by 33 +/- 8%, and low partial derivative tau/partial derivative t downregulated MCP-1 expression by 15 +/- 4%, but oscillating flow upregul ated MCP-1 by 13 +/- 5%. These results suggest that shear stress slew rates regulate monocyte binding by modulating the expression of a potent monocyt e chemoattractant.