Genome scan for quantitative trait loci linked to high-density lipoproteincholesterol - The NHLBI family heart study

We conducted a genome-wide linkage scan for quantitative trait loci influen cing total HDL-cholesterol (HDL-C) concentration in a sample of 1027 whites from 101 families participating in the NHLBI Family Heart Study. To maximi ze the relative contribution of genetic components of variance to the total variance of HDL-C, the HDL-C phenotype was adjusted for age, age(2), body mass index, and Family Heart Study field center, and standardized HDL-C res iduals were created separately for men and women. All analyses were complet ed by the variance components method, as implemented in the program GENEHUN TER using 383 anonymous markers typed at the NHLBI Mammalian Genotyping Ser vice in Marshfield, Wis. Evidence for linkage of residual HDL-C was detecte d near marker D5S1470 at location 39.9 cM from the p-terminal of chromosome 5 (LOD=3.64). Suggestive linkage was detected near marker D13S1493 at loca tion 27.5 cM on chromosome 13 (LOD=2.36). We conclude that at least 1 genom ic region is likely to harbor a gene that influences interindividual variat ion in HDL cholesterol.