Effect of vitamin e modified cellulose membrane on human lymphocyte, monocyte, and granulocyte CD11b /CD18 adhesion molecule expression during hemodialysis

Chronic renal failure induces a clinical state of immunodeficiency that als o depends upon a wide spectrum of dialysis membranes used during hemodialys is. Previous studies have shown that cellular immunodeficiency is caused by malfunction of the antigen presenting cells (monocytes or granulocytes). S ubsequent activation of rolling mononuclear leukocytes results in up-regula ted expression of CD11b/CD18 (Mac-1) on endothelial cells. It is postulated that a VitE coated dialysis membrane might minimize the membrane biocompat ibility, thereby generating a smaller amount of reactive oxygen species (RO S). The purpose of this study was to evaluate the expression of the CD11b/C D18 adhesion molecule on lymphocytes, monocytes, and granulocytes during HD in 10 patients, using flow cytometric analysis. The study protocol include d the measurement of molecule expression using cellulose membrane (Clirans RS15, TERUMO Corp., Japan), and the same membrane coated by vitamin E (Exce brane, Clirans E15, TERUMO Corp., Japan) during 20 dialysis sessions each. Lymphocyte CD11b/CD18 (Mac-1) expression did not change with either dialyze r type. However, monocyte (p = 0.046) and granulocyte (p = 0.018) CD11b/CD1 8 expression in the post HD period was significantly lower using the vitami n E coated membrane compared with the control cellulose membrane. Our findi ngs suggest a significant decrease in activation and migration of monocytes and granulocytes when using a vitamin E coated cellulose membrane.