Gb. Sala-newby et Ac. Newby, Cloning of a mouse cytosolic 5 '-nucleotidase-I identifies a new gene related to human autoimmune infertility-related protein, BBA-GENE ST, 1521(1-3), 2001, pp. 12-18
Citations number
11
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
Adenosine production catalysed by cytosolic 5'-nucleotidase (cN-I) regulate
s diverse physiological processes. We report here a mouse cN-I (mcN-I) clon
ed from heart and testis. The open reading frame contains several potential
translation initiation sites. which yield similarly active 5'-nucleotidase
s. Using overexpression in COS-7 cells we showed that mcN-I, like the previ
ously cloned pigeon cN-I, is activated by ADP and catalyses adenosine forma
tion during ATP breakdown. The N- and C-termini of mcN-I and pcN-I are dive
rgent. Deletion of the 12 C-terminal amino acids or the first 19 N-terminal
amino acids of pcN-I does not diminish activity, although deletion of the
first 31 N-terminal amino acids reduces activity by 70%. Overall mcN-I is o
nly 66% identical to pcN-I or the recently cloned human cN-I (hcN-I), while
hcN-I and pcN-I are 85% identical. We report here a partial hcN-I sequence
that is only 70% identical with the published hcN-I amino acid sequence bu
t is 87% identical with mcN-I. Both hcN-I sequences have perfect matches to
distinct human genome sequences. Our data imply the existence of at least
two genes for cN-I, cN-I-A, previously cloned from pigeon and human. and cN
-I-B that we report here from mouse and partially from human. (C) 2001 Else
vier Science B.V. All rights reserved.