H. Pettersson et G. Pettersson, Kinetics of the coupled reaction catalysed by a fusion protein of beta-galactosidase and galactose dehydrogenase, BBA-PROT ST, 1549(2), 2001, pp. 155-160
Citations number
15
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
The mechanistic implications of the kinetic behaviour of a fusion protein o
f beta -galactosidase and galactose dehydrogenase have been analysed in vie
w of predictions based on experimentally determined kinetic parameter value
s for the galactosidase and dehydrogenase activities of the protein. The re
sults show that the time course of galactonolactone formation from lactose
in the coupled reaction catalysed by the fusion protein can be most satisfa
ctorily accounted for in terms of a free-diffusion mechanism when considera
tion is given to the mutarotation of the reaction intermediate galactose. I
t is concluded that no tenable kinetic evidence is available to support the
proposal that the fusion protein catalyses galactonolactone formation from
lactose by a mechanism involving channelling of galactose. (C) 2001 Publis
hed by Elsevier Science B.V.