Neurocalcin-cactin interaction

Neurocalcin is an N-myristoylated calcium-binding protein which belongs to a novel family of neuronal calcium sensors. Here we show, by cosedimentatio n, co-immunoprecipitation and cross-linking approaches, that myristoylated neurocalcin directly interacts with actin in a calcium-dependent manner. We used EDC cross-linking and obtained one novel 64 kDa entity composed of on e actin molecule and one neurocalcin molecule, as demonstrated with IAEDANS -actin and neurocalcin-specific antibodies. This interaction could modulate the rod outer segment-guanylate cyclase 1-neurocalcin interface. (C) 2001 Elsevier Science B.V. All rights reserved.