Protein kinase C involvement in aloe-emodin- and emodin-induced apoptosis in lung carcinoma cell

1 This study demonstrated aloe-emodin- and emodin-induced apoptosis in lung carcinoma cell lines CH27 (human lung squamous carcinoma cell) and H460 (h uman lung non-small cell carcinoma cell). Aloe-emodin- and emodin-induced a poptosis was characterized by nuclear morphological changes and DNA fragmen tation. 2 During apoptosis, an increase in cytochrome c of cytosolic fraction and a ctivation of caspase-3, identified by the cleavage of its proform, were obs erved. 3 To elucidate whether the expression of protein kinase C (PKC) isozymes ar e involved in aloe-emodin- and emodin-induced apoptosis, this study examine d the changes of PKC isozymes by Western blotting techniques during aloe-em odin- and emodin-induced apoptosis. 4 The expression of PKC isozymes involved in aloe-emodin- and emodin-induce d apoptosis of CH27 and H460 cells. In this study, aloe-emodin and emodin i nduced the changes of each of PKC isozymes in C1127 and H460 cells. 5 The decrease in the expression of PKC delta and epsilon may play a critic al role in aloe-emodin- and emodin-induced apoptosis in CH27 and H460 cells . 6 The present study also demonstrated that PKC stimulation occurs at a site downstream of caspase-3 in the emodin-mediated apoptotic pathway.