Studies on the electron transfer for reaction center isolated from Rhodobacter sphaeroides in self-assembly composite film

The composite ordered protein film was assemblied on the surface of a gold electrode by self-assembly mono-layer approach using meso-2, 3-dimercaptosu ccinic acid (DMSA), poly-dimethyldiallylammonium chloride (PDDA) and reacti on center membrane protein purified from purple bacteria (Rhodobacter sphae roides). Square wave voltammetry(SWV) was employed to study the electrochem ical behavior of the protein composite film. The reversible or quasi-revers ible electron transfer for redox couples therein was successfully detected. The reduction potential for each redox couple and the electrochemically-dr iven effect was also discussed. The typical SWV results were non-linearly f itted to get the kinetic parameters for the composite protein film.