Preparation of ultrathin multilayer films containing poly (4-vinylpyridine) complex of osmium and horseradish peroxidase and its electrochemical behavior

We prepared a new kind of multilayer films based on ionic self-assembly tec hnique by successive alternating deposition of positively charged polycatio n-bearing Os complex(PVP-Os) and horseradish peroxidase (HRP) on the surfac e modified of gold electrode to study the electrochmical behavior and quart z slides to monitor the assembly process by ultraviolet-visible spectroscop y. The electrochemical behavior of the enzyme electrode showed that the mul tilayer films were stable, electroactive, the mediator PVP-Os as electron s huttle was able to transfer electrons successfully between the active cente r of the immobilized HRP and the electrode surface.