In industrial activities, operation of air biofilters may be interrupted fo
r a long period of time. In order to examine the effect of shut-down on the
performance of biofilters, a pilot scale biofiltration unit was restarted
up after a non-use duration of eight months. After a reacclimation period o
f eight days, the biofilter reached an elimination capacity of 35 g.m(-3).h
(-1) compared to 38 g.m(-3).h(-1) before shut-down, for the same operating
conditions. The results obtained indicate that the microorganism activity w
as completely restored and this was confirmed by the microbial count tests
performed on filter bed material samples. The impact of varying the inlet c
oncentration of toluene on biofilter performance was also investigated. A m
aximum elimination capacity of 120 g.m(-3).h(-1) was achieved, compared to
135 g.m(-3).h(-1) before shut-down. The biofilter removal efficiency exceed
ed 96% for inlet concentrations up to 1.8 g.m(-3);. Temperature measurement
s revealed that the filter bed temperature depends strongly on the intensit
y of microbial activity and the biofilter effectiveness in eliminating tolu
ene. Experimental data were compared with the calculations of a mathematica
l model. Comparison showed that the theoretical model was successful in pre
dicting the performance of the biofiltration unit.