Synthesis of porous macrospheres from amino-polymers

Porous macrospheres with controlled diameter were produced using N-methylol acrylamide and urea-formaldehyde resins synthesized in our laboratories. Ho t silicon oil was used to form the porous macrospheres which were character ized by using infrared spectroscopy (FTIR), scanning electron microscopy, m ercury porosimetry and surface area determination (BET). Porous macrosphere s of amino-polymers were used as support in immunoassays type ELISA (enzyme linked immunosorbent assay) in which mouse immunoglobulin (IgG) was immobi lized, and detected by rabbit anti-mouse immunoglobulin (a-IgG) labeled wit h horseradish peroxidase. Responses were obtained up to a dilution ratio of 1:4000 of the conjugate standard (1 mg/ml) and 10 mug/ml of IgG solution t o recover the spheres in pH 4.5. (C) 2001 Elsevier Science Ltd. All rights reserved.