Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative study

Objective: To compare two commercially available sequential media, G1.2/G2. 2 and Sydney IVF cleavage/ blastocyst media, as supports for human embryo c ulture. Design: Prospective randomized study. Setting: University-based IVF clinic. Patient(s): Two hundred forty-nine patients undergoing IVF treatment for th e first or second time, randomly allocated at the time of oocyte retrieval, to either culture in G1.2/G2.2 or Sydney IVF media. Intervention(s): Oocyte recovery, IVF or intracytoplasmic sperm injection, embryo culture, transfer on day 3 or day 5/6. Main Outcome Measure(s): Developmental stage on day 3, blastocyst rate, pre gnancy outcome as assessed by beta hCG positive test, implantation rates, a nd ongoing pregnancies. Result(s): Embryos cultured in G1.2/G2.2 media displayed a faster kinetics of cleavage, compaction, blastulation, and hatching, but a lower day 3 embr yo quality than those grown in Sydney IVF media. For patients with at least five embryos, G1.2/G2.2 media yielded higher implantation rates (26.2%) in our day 3 embryo transfer program when compared to Sydney IVF medium (15.5 %), whereas similar implantation rates were obtained for day 5/6 embryo tra nsfer for both media (43.1% and 36.1%, respectively). Conclusion(s): In our day 3 embryo transfer program, G1.2/G2.2 media were s uperior to Sydney IVF media, whereas both media yielded similar outcomes in our blastocyst transfer program. (C) 2001 by American Society for Reproduc tive Medicine.